How to use the salt corrected melting temperature calculator
Adjust a DNA melting temperature for the sodium ion concentration of the buffer. Enter melting temperature at 1 m sodium, sodium ion concentration in the fields above; the salt corrected tm is computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “melting temperature salt correction”, “Tm sodium correction”, “primer Tm with salt”, “calculate salt corrected melting temperature”, “find salt corrected tm”.
Formula
Variables
- Tm — Melting temperature at 1 M sodium (°C)
- Na — Sodium ion concentration (M)
Worked example
With melting temperature at 1 m sodium = 60 °C, sodium ion concentration = 0.05 M, the calculator gives salt corrected tm ≈ 38.4029 °C.
Frequently asked questions
What is the salt corrected melting temperature calculator used for?
Adjust a DNA melting temperature for the sodium ion concentration of the buffer. It helps with questions such as “melting temperature salt correction”, “Tm sodium correction”, “primer Tm with salt”.
What is the formula for the salt corrected melting temperature calculation?
Salt corrected Tm (°C) = Tm+16.6 × log10(Na). Here Tm is melting temperature at 1 m sodium (°C), Na is sodium ion concentration (M).
What inputs do I need for the salt corrected melting temperature calculator?
You need melting temperature at 1 m sodium in °C, sodium ion concentration in M. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the salt corrected melting temperature calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the salt corrected melting temperature result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.