How to use the dna concentration from a260 calculator
Convert absorbance at 260 nm to DNA concentration. Enter a260 absorbance, dilution factor, conversion in the fields above; the concentration, concentration are computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- A — A260 absorbance
- df — Dilution factor
- f — Conversion (ng/µL per A260)
Worked example
With a260 absorbance = 0.5, dilution factor = 10, conversion = dsDNA, the calculator gives concentration ≈ 250 ng/µL; concentration ≈ 250 µg/mL.
Frequently asked questions
What is the formula for the dna concentration from a260 calculation?
Concentration (ng/µL) = A × df × f. Concentration (µg/mL) = [Concentration]. Here A is a260 absorbance, df is dilution factor, f is conversion (ng/µL per A260).
What inputs do I need for the dna concentration from a260 calculator?
You need a260 absorbance, dilution factor, conversion in ng/µL per A260. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.