How to use the pcr amplification calculator
Compute product copies after PCR cycles. Enter starting copies, cycles, efficiency in the fields above; the final copies, fold amplification are computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- N0 — Starting copies
- c — Cycles
- eff — Efficiency (%)
Worked example
With starting copies = 100, cycles = 30, efficiency = 95 %, the calculator gives final copies ≈ 5.0239 × 10¹⁰; fold amplification ≈ 502,386,900.
Frequently asked questions
What is the formula for the pcr amplification calculation?
Final copies = N0 × (1+eff / 100)^c. Fold amplification = [Final copies] / N0. Here N0 is starting copies, c is cycles, eff is efficiency (%).
What inputs do I need for the pcr amplification calculator?
You need starting copies, cycles, efficiency in %. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.