How to use the primer working solution volume calculator
Find how much primer stock and water to add to make a working primer dilution in a PCR reaction. Enter primer stock concentration, primer working concentration, final reaction volume in the fields above; the primer stock to add, water to add are computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “primer dilution volume”, “primer stock to working”, “PCR primer volume”, “calculate primer working solution volume”, “find primer stock to add”.
Formula
Variables
- Cs — Primer stock concentration (µM)
- Cw — Primer working concentration (µM)
- Vr — Final reaction volume (µL)
Worked example
With primer stock concentration = 100 µM, primer working concentration = 10 µM, final reaction volume = 50 µL, the calculator gives primer stock to add ≈ 5 µL; water to add ≈ 45 µL.
Frequently asked questions
What is the primer working solution volume calculator used for?
Find how much primer stock and water to add to make a working primer dilution in a PCR reaction. It helps with questions such as “primer dilution volume”, “primer stock to working”, “PCR primer volume”.
What is the formula for the primer working solution volume calculation?
Primer stock to add (µL) = Cw × Vr / Cs. Water to add (µL) = Vr-[Primer stock to add]. Here Cs is primer stock concentration (µM), Cw is primer working concentration (µM), Vr is final reaction volume (µL).
What inputs do I need for the primer working solution volume calculator?
You need primer stock concentration in µM, primer working concentration in µM, final reaction volume in µL. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the primer working solution volume calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the primer working solution volume result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.