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DNA GC Content Calculator

Find GC percentage, length and melting temperature of a DNA sequence.

Inputs

Results

Length14bases
GC content50%
Melting temperature37.37143°C
Molecular weight4,278.85g/mol

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How to use the dna gc content calculator

Find GC percentage, length and melting temperature of a DNA sequence. Enter dna sequence in the field above; the length, gc content, melting temperature, molecular weight are computed instantly and plotted on the chart so you can see how the answer changes.

Formula

Length (bases) = clean(seq).length
GC content (%) = gcPct(seq)
Melting temperature (°C) = tm(seq)
Molecular weight (g/mol) = dnaMass(seq)

Variables

  • seq — DNA sequence

Worked example

With dna sequence = ATGCGTACGTTAGC, the calculator gives length ≈ 14 bases; gc content ≈ 50 %; melting temperature ≈ 37.37143 °C; molecular weight ≈ 4,278.85 g/mol.

Frequently asked questions

What is the formula for the dna gc content calculation?

Length (bases) = clean(seq).length. GC content (%) = gcPct(seq). Melting temperature (°C) = tm(seq). Molecular weight (g/mol) = dnaMass(seq). Here seq is dna sequence.

What inputs do I need for the dna gc content calculator?

You need dna sequence. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.

How do I use this calculator?

Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.

Is the result exact?

Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.

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