How to use the qpcr amplification efficiency calculator
Find the PCR amplification efficiency from the slope of a qPCR standard curve. Enter standard curve slope in the field above; the pcr efficiency (fraction), pcr efficiency are computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “qPCR efficiency”, “standard curve slope”, “PCR efficiency percent”, “calculate qpcr amplification efficiency”, “find pcr efficiency”.
Formula
Variables
- slope — Standard curve slope
Worked example
With standard curve slope = -3.32, the calculator gives pcr efficiency (fraction) ≈ 1.000805; pcr efficiency ≈ 100.0805 %.
Frequently asked questions
What is the qpcr amplification efficiency calculator used for?
Find the PCR amplification efficiency from the slope of a qPCR standard curve. It helps with questions such as “qPCR efficiency”, “standard curve slope”, “PCR efficiency percent”.
What is the formula for the qpcr amplification efficiency calculation?
PCR efficiency (fraction) = 10^(-1 / slope)-1. PCR efficiency (%) = 100 × [PCR efficiency (fraction)]. Here slope is standard curve slope.
What inputs do I need for the qpcr amplification efficiency calculator?
You need standard curve slope. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the qpcr amplification efficiency calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the qpcr amplification efficiency result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.