How to use the blue white screening calculator
Find the percent of recombinant colonies from blue and white colony counts in a cloning experiment. Enter white colonies, blue colonies in the fields above; the recombinant (insert-containing) fraction, blue colonies fraction are computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “blue white screening”, “recombinant colonies percent”, “white colony fraction”, “calculate blue white screening”, “find recombinant fraction”.
Formula
Variables
- white — White colonies (colonies)
- blue — Blue colonies (colonies)
Worked example
With white colonies = 40 colonies, blue colonies = 10 colonies, the calculator gives recombinant (insert-containing) fraction ≈ 80 %; blue colonies fraction ≈ 20 %.
Frequently asked questions
What is the blue white screening calculator used for?
Find the percent of recombinant colonies from blue and white colony counts in a cloning experiment. It helps with questions such as “blue white screening”, “recombinant colonies percent”, “white colony fraction”.
What is the formula for the blue white screening calculation?
Recombinant (insert-containing) fraction (%) = white / (white+blue) × 100. Blue colonies fraction (%) = 100-[Recombinant (insert-containing) fraction]. Here white is white colonies (colonies), blue is blue colonies (colonies).
What inputs do I need for the blue white screening calculator?
You need white colonies in colonies, blue colonies in colonies. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the blue white screening calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the blue white screening result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.