How to use the ligation insert:vector ratio calculator
Find how much insert DNA to add for a molar ratio. Enter vector mass, vector length, insert length, molar ratio insert:vector in the fields above; the insert mass needed is computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- vm — Vector mass (ng)
- vl — Vector length (bp)
- il — Insert length (bp)
- r — Molar ratio insert:vector
Worked example
With vector mass = 50 ng, vector length = 3000 bp, insert length = 1000 bp, molar ratio insert:vector = 3, the calculator gives insert mass needed ≈ 50 ng.
Frequently asked questions
What is the formula for the ligation insert:vector ratio calculation?
Insert mass needed (ng) = vm × il / vl × r. Here vm is vector mass (ng), vl is vector length (bp), il is insert length (bp), r is molar ratio insert:vector.
What inputs do I need for the ligation insert:vector ratio calculator?
You need vector mass in ng, vector length in bp, insert length in bp, molar ratio insert:vector. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.