How to use the enzyme activity calculator
Compute enzyme units and specific activity. Enter product formed per minute, enzyme volume, protein amount in the fields above; the activity, total activity, specific activity are computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- dA — Product formed per minute (µmol/min)
- V — Enzyme volume (mL)
- P — Protein amount (mg)
Worked example
With product formed per minute = 2.4 µmol/min, enzyme volume = 0.1 mL, protein amount = 0.5 mg, the calculator gives activity ≈ 24 U/mL; total activity ≈ 2.4 U; specific activity ≈ 4.8 U/mg.
Frequently asked questions
What is the formula for the enzyme activity calculation?
Activity (U/mL) = dA / V. Total activity (U) = dA. Specific activity (U/mg) = dA / P. Here dA is product formed per minute (µmol/min), V is enzyme volume (mL), P is protein amount (mg).
What inputs do I need for the enzyme activity calculator?
You need product formed per minute in µmol/min, enzyme volume in mL, protein amount in mg. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.