How to use the pcr master mix scale-up calculator
Find the total master mix volume for several reactions, including extra volume for pipetting loss. Enter volume per reaction, number of reactions, extra volume for pipetting loss in the fields above; the total master mix volume are computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “master mix calculator”, “scale up PCR mix”, “reagent volume for many reactions”, “calculate pcr master mix scale-up”, “find total master mix volume”.
Formula
Variables
- vr — Volume per reaction (µL)
- n — Number of reactions
- ov — Extra volume for pipetting loss (%)
Worked example
With volume per reaction = 20 µL, number of reactions = 12, extra volume for pipetting loss = 10 %, the calculator gives total master mix volume ≈ 264 µL; total master mix volume ≈ 0.264 mL.
Frequently asked questions
What is the pcr master mix scale-up calculator used for?
Find the total master mix volume for several reactions, including extra volume for pipetting loss. It helps with questions such as “master mix calculator”, “scale up PCR mix”, “reagent volume for many reactions”.
What is the formula for the pcr master mix scale-up calculation?
Total master mix volume (µL) = vr × n × (1+ov / 100). Total master mix volume (mL) = [Total master mix volume] / 1000. Here vr is volume per reaction (µL), n is number of reactions, ov is extra volume for pipetting loss (%).
What inputs do I need for the pcr master mix scale-up calculator?
You need volume per reaction in µL, number of reactions, extra volume for pipetting loss in %. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the pcr master mix scale-up calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the pcr master mix scale-up result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.