How to use the serial dilution calculator
Compute concentration after repeated dilutions. Enter starting concentration, dilution factor per step, number of steps in the fields above; the final concentration, total dilution are computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- C0 — Starting concentration
- DF — Dilution factor per step
- n — Number of steps
Worked example
With starting concentration = 1000, dilution factor per step = 10, number of steps = 6, the calculator gives final concentration ≈ 0.001; total dilution ≈ 1,000,000.
Frequently asked questions
What is the formula for the serial dilution calculation?
Final concentration = C0 / DF^n. Total dilution = DF^n. Here C0 is starting concentration, DF is dilution factor per step, n is number of steps.
What inputs do I need for the serial dilution calculator?
You need starting concentration, dilution factor per step, number of steps. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.