How to use the michaelis–menten enzyme kinetics calculator
Find reaction velocity at a substrate concentration. Enter vmax, km, substrate concentration in the fields above; the reaction velocity, percent of vmax are computed instantly and plotted on the chart so you can see how the answer changes.
Formula
Variables
- Vmax — Vmax (µmol/min)
- Km — Km (mM)
- S — Substrate concentration (mM)
Worked example
With vmax = 100 µmol/min, km = 2 mM, substrate concentration = 5 mM, the calculator gives reaction velocity ≈ 71.42857 µmol/min; percent of vmax ≈ 71.42857 %.
Frequently asked questions
What is the formula for the michaelis–menten enzyme kinetics calculation?
Reaction velocity (µmol/min) = Vmax × S / (Km+S). Percent of Vmax (%) = 100 × [Reaction velocity] / Vmax. Here Vmax is vmax (µmol/min), Km is km (mM), S is substrate concentration (mM).
What inputs do I need for the michaelis–menten enzyme kinetics calculator?
You need vmax in µmol/min, km in mM, substrate concentration in mM. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use this calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.